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KMID : 1377020130100040183
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Tissue Engineering and Regenerative Medicine
2013 Volume.10 No. 4 p.183 ~ p.191
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Improvement of osteoblast functions by sustained release of bone morphogenetic protein-2 (BMP-2) from heparin-coated chitosan scaffold
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Yun Young-Pil
Lee Su-Young
Kim Hak-Jun
Song Jae-Jun
Kim Sung-Eun
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Abstract
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The aim of this study was to investigate the improvement in osteoblast functions by using bone morphogenetic protein-2 (BMP-2) immobilized heparin-coated chitosan scaffolds and comparing it with that using chitosan scaffold or BMP-2/chitosan scaffold in vitro. BMP-2 was released from the heparin-coated chitosan scaffold in a sustained manner compared to that released from the chitosan scaffold. The osteoblast functions of MG-63 cells grown on the chitosan scaffold, the BMP-2/chitosan scaffold, the BMP-2/Hep/chitosan scaffold were investigated by assessing cell proliferation, alkaline phosphatase (ALP) activity, calcium deposition, and gene expression. The results of the in vitro studies demonstrated that MG-63 cells grown on the BMP-2/Hep/chitosan scaffold showed a significant increment in ALP activity, and calcium deposition as compared to those grown on the chitosan scaffold by sustained release of BMP-2 due to the influence of heparin. Therefore, BMP-2 immobilized heparin-coated chitosan scaffolds are promising materials for improving osteoblast functions through sustained release of BMP-2.
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KEYWORD
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bone morphogenetic protein-2 (BMP-2), heparin, osteoblast function, sustained release, chitosan scaffold
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